NovaSeq™ X Plus is Illumina’s latest high-throughput sequencing system that delivers exceptional performance in terms of throughput, production capacity and sustainability. With a throughput of up to 2.5 times greater than previous sequencers, it can generate more than 20,000 whole genomes annually and provide an ideal choice for large-scale sequencing projects and ambitious genomics studies. Validation run with PhiX library and human whole genome sequencing are completed. The results are available now! Let us discover the extraordinary capabilities of the cutting-edge solutions. Try a NovaSeq X Plus lane with Novogene！

1. Quality Control Overview of Validation Testing with PhiX Library

The average data output and Q30 are 443Gb/lane and >95%, respectively. One lane highly reached 465Gb.

2. Testing Result of Human Whole Genome Sequencing

Using sample NA12878 for library preparation, performing whole-genome sequencing simultaneously on the NovaSeq X Plus and NovaSeq 6000 to further evaluate the platform performance.

2.1 Quality Control

Compared with the NovaSeq 6000, the Novaseq X Plus platform performs better in Q30 (94.63% in average).

2.2 Mapping Statistics

The Novaseq X Plus and NovaSeq 6000 platforms achieve even coverage across the entire genome with coverage of ~99% under an average sequencing depth of 30x.

2.3 Variant Calling Accuracy

The NovaSeq X Plus and NovaSeq 6000 have >95% similarity in SNP detection. The SNP and InDel precision and recall deviation between the two platforms is <0.2%.

Figure 1 Venn diagrams of shared SNPs between NovaSeq X Plus and NovaSeq 6000

High data concordance between samples sequenced on separate lanes was observed on the NovaSeq X Plus and NovaSeq 6000 platforms. This demonstrates the high reproducibility of variant detections between sequencing runs and the high data quality achievable with our workflow on the latest sequencing platforms.

About Novogene

Novogene is a pioneer in applying cutting-edge molecular biology technology and high-performance computing to research in the fields of life science and human health. With one of the largest sequencing capacities in the world, we utilise our deep scientific knowledge, first-class customer service and unsurpassed data quality to help clients realise their research goals in the rapidly evolving world of genomics. Novogene is committed to become your trusted genomics partner.