ATAC-seq is a high-throughput sequencing method used to analyze genome-wide chromatin accessibility, which is crucial for understanding the global epigenetic regulation of gene expression. Chromatin accessibility refers to the extent to which nuclear macromolecules can interact with chromatinized DNA, enabling transcription and other biological processes. Consequently, ATAC-seq provides valuable insights into the genome’s regulatory landscape by identifying regions where DNA is accessible for binding by various cellular factors.
Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq）
Benefits of Assay for Transposase-Accessible Chromatin with Sequencing (ATAC-seq）
Flexible Sample Receiving Strategies
Accepts both cells and tissues with low starting amounts.
Profound Experience
Successfully completed hundreds of ATAC-seq projects.
Rapid and High Capacity
Superior turnaround efficiency.
Comprehensive Bioinformatic Analysis
Provides publication-ready results and offers bioinformatic consultants for ATAC-seq data interpretation.
US-based processing
Library preparation, sequencing and customer support in California, Davis.
Applications
Capturing all open chromatin regions under specific space-time conditions, designed to evaluate genome-wide regulatory potential of research goals:
Deciphering Fundamental Biology
Profiling open chromatin in different cell state to decipher cell fate change during cell deveopment, cell differentiate and cellular response.
Understanding Disease Mechanisms
Linking genetic variants to regulatory dysfunction, exploring gene functions in disease progression.
Advancing Drug Discovery & Therapeutics
Profile drug-triggered chromatin regulation to decipher drug efficacy and resistance. Disease-or drug-specific chromatin signatures act as diagnostic and prognostic biomarkers.
Specifications
Sample Requirements
Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.
Refer to Data standards – ENCODE for more information about data amount and analysis process for ATAC-seq.
Sequencing and Analysis
Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.
Project Workflow of Novogene ATAC-seq Service
The Novogene ATAC-seq service comprises four steps: Sample preparation, library preparation, sequencing, and bioinformatics analysis. The workflow cannot be paused after sample thawing due to the nature of the initial samples (cells/tissues). Please contact us for more information about your ATAC-seq projects.
To guarantee the accuracy and reliability of sequencing data, Novogene audits every experimental step through quality control, fundamentally ensuring high-quality data output from sampling to the final data report. This commitment to high-quality data is essential for the correctness, comprehensiveness, and credibility of bioinformatics analysis.
Resources
Demo Results
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Distribution of the Reads Mapped to the Gene Body
Horizontal axis: relative position of the gene. Vertical axis reads density.
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Genome Wide Distribution of Peaks
Horizontal axis is the coordinate of the peak in the chromosome. Vertical is the chromosome. Every blue line represents a peak.
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Motif Analysis
The most significant 25 motifs of length 8, 10, 12, 14 were identified for each set of experiments. More detailed information for each motif is available in the result file and the readme file.
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Distribution of Peaks in Functional Regions
Distribution of peak in different
functional area.
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Enrichment Analysis
Left is a demo graph of GO term enrichment. Right is a demo graph of KEGG pathway enrichment.
Webinars
Introduction to ChIP-seq and ATAC-seq Services: Unveiling Epigenetic Multi-Omics at Novogene
In this webinar, Novogene will provide a comprehensive overview of two powerful epigenetic sequencing products offered by Novogene: ChIP-seq and ATAC-seq. Our expert speaker will discuss the capabilities of these technologies and their potential applications in the field of epigenetics. In addition, Novogene will also present our bioinformatic analysis services, which utilize industry-standard software and programs to provide motif prediction, peak annotation, functional analysis, and data visualization.
NGS Guide – ChIP-Seq and ATAC-Seq
Genome-wide chromatin accessibility is essential for global epigenetic control of gene expression. ATAC-seq is a high-throughput sequencing method for the study of chromatin accessibility. ChIP-Seq combines the selectivity of ChIP with the power of next-generation sequencing (NGS), providing genome-wide profiling of DNA targets for DNA-associated proteins. Our services provide comprehensive solutions starting from library construction to data analysis. Through this webinar, you will be introduced to how these technologies can power your gene expression regulation research and serve as a guide for such sequencing services.
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