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Full-Length Transcriptome Sequencing

Comprehensive full-length transcript sequencing to resolve transcript structures, isoform diversity, and gene expression complexity.

Full-length transcriptome sequencing provides end-to-end coverage of transcripts, enabling accurate isoform profiling, alternative splicing analysis, detection of fusion transcripts, and discovery of novel RNA species. Leveraging PacBio SMRT long-read sequencing, researchers can obtain high-confidence, assembly-free full-length cDNA reads for precise characterization of transcript diversity across medical, agricultural, and functional genomics studies. In parallel, Oxford Nanopore direct RNA Sequencing captures continuous, native RNA molecules without reverse transcription or PCR, preserving RNA modifications, poly(A) tail length, and structural information to reveal post-transcriptional regulation with exceptional depth.


With extensive long-read sequencing experience, advanced PacBio and Nanopore platforms, and rigorous quality standards, Novogene delivers highly accurate, reproducible, and comprehensive full-length transcriptome data.

Why Choose Novogene for Full-Length Transcriptome Sequencing?

Direct Profiling of Native RNA Modifications
Direct Profiling of Native RNA Modifications

Direct RNA sequencing bypasses cDNA conversion to directly detect base modifications and poly(A) tail lengths, providing an unbiased view of the native transcriptome and complementing isoform sequencing.

Comprehensive & Reproducible Analytical Workflows
Comprehensive & Reproducible Analytical Workflows

We employ a well-developed, proprietary pipeline for end-to-end analysis. This ensures robust discovery of novel transcripts, accurate differential expression, and functional annotation from your full-length data.

High-Throughput Capacity & Cost-Effective Service
High-Throughput Capacity & Cost-Effective Service

We leverage leading sequencing infrastructure and efficient project management for fast turnaround times. This integrated approach delivers reliable, large-scale solutions at accessible prices.

Applications of Full-Length transcriptome Sequencing in Research and Medicine

Discover the power of full-length transcriptome sequencing with Novogene's services, delivering unbiased and complete RNA profiles to unlock groundbreaking discoveries.

Discover Novel Transcripts and Alternative Splicing Events

Identify and characterize previously unknown genes, isoforms, and fusion transcripts. Comprehensively profile alternative splicing events to uncover potential biomarkers.

Decipher Disease Mechanisms & Drug Resistance

Investigate the molecular basis of diseases by linking full-length transcripts to pathology.

Uncover transcriptome-level changes that drive drug resistance in cancer and infectious diseases.

Profile RNA Modifications & Regulation

Directly detect epitranscriptomic marks (e.g., m6A) and study their role in gene regulation. Accurately measure poly(A) tail length to understand its impact on RNA stability and translation.

Enhance Genomic Annotation & Evolution

Build high-quality annotations for non-model organisms to support evolutionary studies.

Study Development & Stress Responses

Track dynamic changes in isoform expression throughout developmental processes.

Understand plant and animal responses to biotic and abiotic environmental stresses.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample TypeServiceAmount (Qubit)ConcentrationRIN (Agilent 2100)Purity(NanoDrop™)
TotaI RNAFull-Length Transcriptome Sequencing (PacBio)≥ 600 ng≥ 40 ng/μL≥ 6.5 with flat
baseline		OD260/280=1.8-2.2;
OD260/230=1.3-2.5;
NC/QC ≤ 2;
No genomic
contamination
TotaI RNADirect RNA (Nanopore)≥ 10 μg≧ 375 ng/μL≥ 7 (animals, plants,fungi);
≥ 8 (Human)OD260/280=1.8-2.2;
OD260/230=1.6-2.5;
NC/QC ≤ 2;
28S/18S, 25S/18S ≥ 0.7;
No gel well contamination / no genomic DNA contamination / no precipitation / no impurities, sample clear and transparent

Sequencing and Analysis

Sequencing PlatformFull-Length Transcriptome Sequencing (PacBio)Direct RNA (Nanopore)
Sequencing Strategy• 5 million HiFi reads for highly expressed genes/isoforms detection;

• 10 million HiFi reads for moderate/rare genes/isoforms detection;

• Genome-assisted annotation for species-wide transcriptome refinement, multiplexing of 4-6 samples per cell for efficient project design.		10M~20M reads/sample
(1 PromethION cell/ sample)
Data Analysis Capability• Data Quality Control

• Transcriptome Analysis

• Isoform Characterization

• Structural Category

• Length Distribution
• Transcriptome Diversity

• Function Annotation

• Structure Analysis

• Transcription Factor analysis

• lncRNA prediction*
• Fusion Transcript analysis*

• Alternative Splicing analysis*

• Alternative PolyAdenylation analysis*

• Quantitative Analysis

• Gene/Transcript Expression Level Analysis

• Differentially Expressed Gene/Transcripts Analysis

• Enrichment analysis of deferentially expressed gene

• GO enrichment analysis of deferentially expressed gene

• KEGG enrichment analysis of deferentially expressed gene		• Full-length transcripts identification and classification*

• Transcript structure analysis*

• Alternative splicing*

• Novel gene/ Novel transcript prediction and annotation*

• Transcription factor analysis*

• LncRNA prediction*
• Quantification analysis*

• Quantification analysis*

• Differential gene expression analysis*

• GO/KEGG enrichment analysis of differentially expressed genes*

• RNA modification analysis*

• Detection of methylation sites and motif analysis (5mC/ m6A)*

• KEGG/GO enrichment analysis*
• Differential methylation site analysis*

• Poly(A) analysis*

Project Workflow

For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.

Project Workflow

Demo Results

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Classification of Full-Length Transcripts

The pie chart below illustrates the categorization of full-length transcripts.

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Alternative Splicing

Overview of alternative splicing events from full-length transcript sequencing data.

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LncRNA Prediction and Analysis

The Venn diagram compares noncoding transcript predictions from multiple tools, highlighting the consensus set chosen for reliable downstream analysis.

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Differential Gene Expression Analysis

The volcano plot summarizes the results of differential expression analysis, immediately highlighting genes with significant expression changes.

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Enrichment Analysis of Differentially Expressed Genes

The bar graph displays the most significantly enriched Gene Ontology terms from the functional enrichment analysis of differentially expressed genes.

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Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.