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Prokaryotic RNA Sequencing

Prokaryotic RNA sequencing employs cutting-edge next-generation sequencing (NGS) technology to systematically profile the transcriptome of prokaryotic organisms, capturing both coding and non-coding RNA transcripts. By utilizing strand-specific RNA library construction, this approach ensures accurate measurement of transcript expression, delivering reliable and precise data.


At Novogene, the advanced Illumina NovaSeq platforms are employed for cDNA library preparation and sequencing. Utilizing paired-end 150-base-pair (150 bp) protocols, these platforms consistently generate high-quality, high-fidelity short-read data. With extensive experience and significant sequencing capacity, Novogene offers a comprehensive and customizable suite of services designed to address diverse and complex research requirements.

Advantages of Novogene's Prokaryotic RNA Sequencing Services

Extensive Expertise
Extensive Expertise

With a proven track record of delivering more than 1 million RNA-seq samples per year, Novogene is a trusted leader in genomic sequencing.

Tailored Solutions
Tailored Solutions

Benefit from customized workflows and specialized analyses designed to address the unique demands of complex or challenging samples.

Exceptional Data QualityExceptional Data Quality
Exceptional Data Quality

Consistently outperforming industry benchmarks, Novogene ensures high-fidelity and accurate data to meet your research objectives.

Applications of Prokaryotic RNA Sequencing Description

Prokaryotic RNA-seq offers a detailed understanding of gene expression and regulatory networks in bacteria and archaea. This powerful tool supports studies in microbial physiology, pathogenesis, and adaptive responses to stress, providing valuable insights into prokaryotic biology.

Environmental-Impact Analysis

Explore how environmental changes influence microbial biological functions and ecological roles.

Stress-Response Mechanisms

Investigate microorganism physiological adaptations under various stress conditions to uncover survival strategies.

Trait-Differentiation Studies

Pinpoint key functional genes responsible for phenotypic variations or differential traits triggered by specific treatments.

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Library TypeSample TypeAmountRNA Integrity NumberPurity
Prokaryotic RNA LibraryTotal RNA≥ 500 ng≥ 6.0, smooth base lineA260/280 ≥ 2.0;
A260/230 ≥ 2.0

Sequencing and Analysis

Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Sequencing PlatformIllumina Sequencing System
Read LengthPaired-end 150bp
Recommended Data Amount≥ 2Gb raw data per sample for the species with reference genome
Content of Data AnalysisData Quality Control
Alignment to Reference Genome
Gene Expression Analysis
Novel Transcript Prediction
Gene expression quantification & Differential expression analysis
Enrichment Analysis
Operon, Promoter and TSS/TTS Prediction
SD Sequence Prediction
Rho-independent Terminator Sequence Prediction
sRNA Secondary Structure Prediction
sRNA Targeted Gene Prediction

Novogene Prokaryotic RNA-seq Workflow

Our Prokaryotic RNA-seq workflow ensures high-quality results through a rigorous, step-by-step process:

Novogene Prokaryotic RNA-seq Workflow

Resources

Background

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