Sequencing-Only on Illumina Sequencer
Novogene provides cost‑effective sequencing‑only services for customer‑prepared, ready‑to‑sequence libraries on the state‑of‑the‑art NovaSeq X Plus platform. With a flexible portfolio of sequencing solutions, we offer multiple read lengths, throughput options, and turnaround times to support a wide range of research needs and project scales.
Our experienced team ensures an efficient, seamless sequencing experience through rigorous quality control, standardized workflows, and extensive project expertise. By combining professional scientific capabilities with optimized operational processes, Novogene delivers reliable, high‑quality data and dependable support for your sequencing projects.
Why Choose Novogene for Your Illumina Sequencing Needs?
Cost-Effective High-Performance Sequencing
Access some of the most competitive sequencing costs on a per-read/per-Gb basis, with a guaranteed Q30 ≥ 85% for high-quality data you can trust.
Trusted Expertise & Professional Support
Rely on Novogene’s experienced team for seamless project handling, technical guidance, and consistent, high-quality sequencing output from start to finish.
Fast, Efficient Turnaround Times
Benefit from Novogene’s rapid, no-extra-fee turnaround, enabling quicker progress from library submission to data delivery and helping you meet demanding project timelines.
Flexible Project Configuration
Choose from multiple lane or data amount purchase options, support for a broad range of library types, and customizable sequencing parameters to fit any project size or design.
Laboratory Network
Enjoy convenience and faster logistics with Novogene’s local laboratory operations across the USA (Davis, CA, and Beaverton, OR)
Automated Workflow & Integrated Project Management
Experience streamlined project execution through Novogene’s Customer Service System (CSS), enabling efficient data management and communication throughout the sequencing process.
Applications of Sequencing-Only Services
High-throughput sequencing of customer-prepared libraries empowers a wide range of research workflows with maximum flexibility, efficiency, and control.
Power Large-Scale and High-Throughput Studies
Generate consistent, high-volume sequencing output for population genetics, clinical research, screening projects, and large cohort studies that require scalable and reliable data generation.
Enhance Custom Bioinformatics Pipelines
Obtain high-quality raw sequencing data designed for seamless integration into your in-house analysis workflows, giving you full control over data processing, interpretation, and downstream analytics.
Optimize Project Design and Budget Allocation
Combine your preferred library preparation methods with Novogene’s high-capacity sequencing to optimize costs, streamline resources, and customize your NGS workflow end-to-end.
Support Broad Omics Research Needs
Enable flexible data generation for genomics, transcriptomics, targeted sequencing, metagenomics, and other NGS-based applications that rely on accurate, scalable sequencing-only solutions.
Specifications
Flexible Partial Lane Sequencing on NovaSeq X Plus
Our Partial Lane Sequencing service allows multiple projects to share a lane on the NovaSeq X Plus, offering economic flexibility and easy access to small data volume options using the adavenced sequencing technology. Your libraries are expertly grouped and balanced with other samples to ensure optimal base diversity on the lane, eliminating the need for PhiX spike-in libraries as balancers and maximizing usable lane space.
Reliable Full Lane Sequencing on NovaSeq X Plus
Our Full Lane Sequencing service offers entire lanes of the flow cell on NovaSeq X Plus for exceptional throughput and sequencing consistency. By minimizing cross-contamination risks, the Full Lane Sequencing ensures reliability and precision of your sequencing results.
To maintain high-quality data from NovaSeq X Plus, it’s essential to preserve base diversity on the lane. We recommend selecting index adapters with diverse index sequences that optimize color balance within pooled libraries. This strategy is essential for successful demultiplexing and subsequent data analysis.
Sequencing and Analysis
Project Workflow
For customer‑prepared libraries, Novogene performs an initial library quality assessment to ensure they meet sequencing requirements. Qualified libraries are then pooled based on effective concentration and requested data volume and loaded onto Illumina sequencers for high‑throughput sequencing.
Demo Results
Quality Score Distribution Along Reads
Quality score distribution across sequencing cycles, reflecting overall base-calling accuracy along read positions.
Error Rate Distribution Along Reads
Base error rate across read positions, illustrating sequencing accuracy and quality stability along the read length.
Base Content Along Reads
Base composition distribution (A, T, G, C) across sequencing cycles to evaluate potential sequence bias.
Classification of Raw Reads
Summary of raw read filtering results, showing proportions of clean reads, adapter-related reads, and other read categories.
Blogs
Brochure
Ready to Start Your Project?
Our platform offers tailored solutions for
your unique experimental needs, ensuring a seamless experience from project design to data delivery.