Illumina Single Cell 3’ RNA Technology employs cell barcoding and UMI-based quantification to efficiently capture poly(A)+ transcripts at single-cell resolution, ensuring precise and reproducible gene expression measurements for each individual cell. Sequencing outputs are processed through the DRAGEN pipeline to produce standardized gene-by-cell expression matrices that are fully compatible with leading downstream analysis platforms such as Seurat and Scanpy.

The Illumina Single Cell platform includes scalable configurations (T2, T10, T20, and T100) to accommodate varying project sizes and throughput requirements. At Novogene, we currently deliver this workflow using the Illumina T10 platform, providing standardized library preparation, scalable throughput, and consistent performance across projects.

To further assess platform performance, Novogene conducted an internal benchmarking study evaluating gene detection sensitivity, UMI distribution, mapping efficiency, and clustering consistency across single-cell platforms. The findings demonstrated strong concordance in transcriptomic resolution and downstream analytical compatibility. Detailed benchmarking results are available in our technical poster.