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Qualitative Proteomics

Comprehensive identification of proteins to characterize proteome composition and explore biological functions and molecular mechanisms.

In modern proteomics, protein identification is fundamental for advancing our understanding of biological systems, disease mechanisms, and therapeutic targets. Tandem Mass Spectrometry (MS/MS) has revolutionized the process of protein identification and analysis by offering precise and high-throughput capabilities to analyze complex protein mixtures with remarkable sensitivity.


At Novogene, our qualitative proteomics platform is powered by Thermo Astral with DIA acquisition mode, significantly enhancing selectivity, accuracy, and throughput. This powerful combination enables comprehensive coverage and highly confident protein identification and characterization across diverse biological systems.

Why Choose Novogene for Your Proteomics Needs?

Seamless from Sample to Insight
Seamless from Sample to Insight

End-to-end proteomics workflow from expert sample prep to LC-MS/MS and data analysis

Customized for Your Success
Customized for Your Success

Optimized pretreatment and high-quality data tailored to qualitative research needs

Beyond Identification: Enabling Insights
Beyond Identification: Enabling Insights

Move beyond mere identification to uncover functional mechanisms with domain and motif analysis in Novogene's reports

Advanced platform
Advanced platform

Thermo Astral with DIA enables near-complete ion use for deeper, more sensitive proteome coverage

Applications

Discover the power of qualitative proteomics with Novogene’s services, designed to assist in a variety of research goals:

Unlocking Biological Mechanisms

Precisely map protein expression, interactions, and structure across complex systems

Supporting Drug Development and Biomarker Discovery

Inform target selection, mechanism of action, and biomarker development

Specifications

Sample Requirements

Sample amounts are listed for reference only. Download the Sample Submission Guidelines to learn more. For detailed information, please contact us with your customized requests.

Sample Type*Sample Amount
Gel fragments (CBB staining, silver staining for mass spectrometry, etc.)Area < 1 cm2 (extra fee for larger areas)
Pure protein (best buffer is 6M Urea)50 μg (concentration > 1 μg/μL)
Protein with loading buffer50 μg (concentration > 1 μg/μL)
Beads (Protein A/G magnetic beads/Agarose beads - antigen-antibody complex)50 μg (estimation)
* For detailed submission guidelines on other sample types, please refer to our quantitative proteomics page or contact us directly.

Detection Details

The Orbitrap Astral high-resolution mass spectrometer comprises three mass analyzers (as shown below). By integrating these analyzers, the system leverages their unique strengths to enhance performance and usability, ultimately improving its overall capabilities.

1. Quadrupole mass analyzer: Enables precise precursor ion selection.

2. Orbitrap mass analyzer: Delivers high-resolution, high dynamic range HRAM (High-Resolution Accurate Mass) spectra.

3. Astral mass analyzer: Provides high-sensitivity detection and rapid acquisition of high dynamic range HRAM spectra at speeds of up to 200 Hz.


Analyzer TypeAstral Mass Analyzer
SensitivitySingle ion detection
HRAM scan rateUp to 200 Hz
Interscan dynamic range>1000 with single microscan
Resolution80,000 at m/z 524; >50,000 at m/z 130 for TMTpro TMT 18-plex
Mass accuracyRMS <5 ppm
Mass rangem/z 40-6,000
Scan modeSIM, DDA, DIA, PRM

Project Workflow

Our protein identification workflow integrates three key components - advanced instrumentation, validated procedures, and expert analysis - to deliver reliable results. The standard procedure is outlined below:

1. Sample Preparation

Protein extraction and cleanup from tissues, cells, body fluids, gel, and so on

2. Protein Digestion

In-solution or on-beads or in-gel trypsion digestion into peptides

3. LC-MSMS detection

Protein identification and sample QC

4. Database Search & Bioinformatics

Spectra searched in Spectronaut for peptide mapping

5. Report & Biological insights

Annotated and comperhensive reports with sequence coverage and functional interpretation

Project Workflow

Resources

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Protein Identification

Understanding which proteins exist in a system

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Protein Functional Annotation

To label each protein with its potential biological roles

Frequently Asked Questions

What you will receive, when you select our protein identification services?

1. A comprehensive report detailing each identified protein with name, accession number, sequence coverage, number of unique peptides and functional annotation.

2. Raw data files also can be provided.

3. A glossary of methods, key instrument parameters and interpretation notes to support publication or internal decision-making.

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Our platform offers tailored solutions for

your unique experimental needs, ensuring a seamless experience from project design to data delivery.